Part:BBa_K3096020

Constitutive nagC expression

This composite part generates nagC. NagC is a repressor for the nag-operon which is needed for GlcNAc6P catabolism. If nagC is bound by GlcNAc6P it looses its repression capability. NagC is an activator for the glm-operon which is responsible for GlcNAc6P anabolism.

Usage and Biology

Commensal bacteria in the gut metabolize the mucus within the intestines, which increases the level of GlcNAc within the lumen. GlcNAc is taken up by the bacteria through their PTS system and metabolized into GlcNAc-6-P, which binds the repressor protein nagC. If nagC is bound to GlcNAc-6-P it loses ist abilities to bind DNA, and therefore ist respective regulation activity. NagC can consequently not serve as a repressor of the pNagC controlled nagBACDE operon, anymore.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Sicard JF, Le Bihan G, Vogeleer P, Jacques M, Harel J. Interactions of Intestinal Bacteria with Components of the Intestinal Mucus. Front Cell Infect Microbiol. (2017); 7:387. Published 2017 Sep 5. doi:10.3389/fcimb.2017.00387
2. Barnhart MM, Lynem J, Chapman MR. GlcNAc-6P levels modulate the expression of Curli fibers by Escherichia coli. J Bacteriol. (2006); 188(14):5212–5219. doi:10.1128/JB.00234-06
3. Konopka JB. N-acetylglucosamine (GlcNAc) functions in cell signaling. Scientifica (Cairo). (2012); 2012:489208. doi:10.6064/2012/489208